Helicobacter pylori with Clarithromycin Resistance Prediction, Molecular Detection, PCR, Varies
Use
This test is used for assessing pure isolates of Helicobacter pylori to predict clarithromycin resistance or susceptibility. It detects the Helicobacter pylori 23S ribosomal RNA gene and the three most common single nucleotide variations (A2143G, A2142G, and A2142C) that lead to resistance to clarithromycin. This helps guide the appropriate antimicrobial therapy for infections caused by this organism.
Special Instructions
Not provided.
Limitations
This assay exclusively examines the three most common 23S ribosomal RNA point variants associated with clarithromycin resistance in Helicobacter pylori. It does not assess other mechanisms of clarithromycin resistance or resistance to non-clarithromycin antimicrobial agents. Cross-reactivity may occur with non-pylori Helicobacter species. Additionally, other clarithromycin resistance mechanisms or resistances to different antimicrobial agents are not determined by this test. Pure cultures are needed as the organism is fastidious once isolated and may not be amenable to phenotypic susceptibility testing.
New York Approved
Methodology
PCR-based (Real-Time PCR)
Biomarkers
No genes
Gene
LOINC Codes
- 88509-5 - H pylori 23S clarithro res mut Islt/Spm
- 31208-2 - Specimen source
- 49101-9 - H pylori DNA Spec Ql NAA+probe
- 88509-5 - H pylori 23S clarithro res mut Islt/Spm
Result Turnaround Time
3-4 days
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
Isolate
Minimum Volume
Not provided
Container
Agar slant or other appropriate media
Collection Instructions
Perform isolation of Helicobacter pylori in culture. Submit the isolate in pure culture. Do not submit mixed cultures.
Storage Instructions
Place specimen in a large infectious container and label as an etiologic agent/infectious substance if appropriate.
Causes for Rejection
Agar plate, ESwab, Port-a-Cult