Hemoglobin F Distribution, Blood
Use
The test is useful for distinguishing large deletional hereditary persistence of fetal hemoglobin (HPFH) from other conditions characterized by increased percentage of fetal hemoglobin (Hb F). It determines the distribution of Hb F within red blood cells, providing insight into conditions such as beta-thalassemia major, sickle cell disease, aplastic anemia, certain leukemias, and hemoglobinopathies associated with hemolysis. This test guides in understanding the physiologic and pathologic increases in Hb F, important in disorders like hereditary spherocytosis and alpha-thalassemia minor.
Special Instructions
This test is not directly orderable and is only available as a reflex. It is specifically designed for hereditary persistence of fetal hemoglobin analysis. For other conditions such as possible fetal-maternal bleed, alternative tests such as Fetomaternal Bleed, Flow Cytometry, Blood are recommended.
Limitations
The test's accuracy for hemoglobin F distribution relies on flow cytometric analysis. Results are relevant when Hb F percentage is between 15% to 35%. Above 35%, clinical correlation is needed for interpretation. Cases with Hb F under 15% may not indicate HPFH, and results above 35% are better approached through molecular and familial studies. It's crucial to quantify Hb F percentage before flow cytometry analysis to evaluate test appropriateness.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 4579-9
- 4579-9
- 59466-3
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
Not provided
Minimum Volume
0.5 mL
Container
EDTA tube
Causes for Rejection
Gross hemolysis, clotted blood
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |