Hepatitis B Virus (HBV) DNA, Quantitative Real-time PCR With Reflex to HBV Genotype
Also known as: HBV Quantitation, HBV Viral Load
Use
This test quantitates HBV DNA in serum or plasma with reflex to HBV genotype if ≥500 IU/mL is detected.
Special Instructions
The test requires plasma or serum samples which must be frozen. It is essential to use a red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or PPT for collection. The sample must be centrifuged within six hours of collection, and the serum/plasma transferred to a screw-top polypropylene tube before freezing. Green-top (heparin) or yellow-top (ACD) tubes are not acceptable for collection.
Limitations
The quantitative assay used in the test has a defined measurable range, and genotyping is only conducted if the viral load exceeds the threshold of 500 IU/mL. Reflex testing is contingent upon the initial test's results, which means the genotyping is not performed if the viral load is below 500 IU/mL. Furthermore, improper sample handling or collection outside of specified guidelines may lead to rejection of the specimen.
Methodology
PCR-based (qPCR)
Biomarkers
LOINC Codes
- 42595-9
- 42595-9
- 48398-2
- 19147-8
Result Turnaround Time
7-9 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
3 mL
Minimum Volume
1.5 mL
Container
Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or PPT
Collection Instructions
Centrifuge sample within six hours of collection. Transfer the serum/plasma to a screw-top polypropylene tube and freeze.
Storage Instructions
Freeze.
Causes for Rejection
Green-top (heparin) or yellow-top (ACD) tubes used for collection; serum/plasma not frozen; PPT not centrifuged
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