HER-2 Immunohistochemistry (IHC), Breast With Reflex to FISH if 2+ by IHC
Use
Tissues are evaluated by immunohistochemistry. Specimens demonstrating 2+ (moderate) staining for the HER-2/neu protein are evaluated for gene amplification status by FISH. In such cases, FISH provides a better indication of potential response to therapy.
Special Instructions
Please direct any questions regarding this test to oncology customer service at 800-345-4363.
Limitations
Use of fixatives other than 10% neutral-buffered formalin or fixation times outside six to 72 hours may yield unreliable results. Decalcification procedures can negatively affect antigenicity and DNA quality.
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 18474-7
- 19147-8
- 77202-0
- 72486-4
Result Turnaround Time
4-5 days
Related Documents
For more information, please review the documents below
Specimen
Tumor Tissue (FFPE)
Volume
One paraffin block or seven unstained, positively-charged tissue slides cut at 4 to 5 microns and dried for one hour at 60°C.
Minimum Volume
Not provided
Container
Paraffin block transport pouch or slide mailer
Collection Instructions
Specimen should be fixed in 10% neutral-buffered formalin. Time from tissue acquisition to fixation should be as short as possible. Fixation time should be between six and 72 hours according to ASCO/CAP guidelines.
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Tumor other than breast tumor
Other tests from different labs that may be relevant