High-risk HPV With HPV Genotypes 16 and 18 (Cobas®)
Use
This test specifically identifies types HPV16 and HPV18 while concurrently detecting the rest of the high-risk types: 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68 without further specific differentiation.
Special Instructions
Not provided.
Limitations
The detection of high-risk HPV is contingent upon the number of viral copies present in the specimen, which may be influenced by collection methods, patient factors, infection stage, and interfering substances. Specimens that are improperly collected or handled, inadequately labeled, or submitted incorrectly can result in rejection. Specimens from male patients or those that are excessively bloody, improperly preserved, or old beyond specified limits will not be accepted.
Methodology
PCR-based (RT-PCR)
Biomarkers
HPV
Microorganism
LOINC Codes
- 71432-9
- 71431-1
- 77399-4
- 77400-0
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Cervical Swab
Volume
2 mL
Minimum Volume
1 mL
Container
ThinPrep® vial or SurePath™ vial
Collection Instructions
Brush/spatula technique: Insert the brush into the endocervical canal until only the bottommost fibers are exposed. Slowly rotate the brush 1/4 to 1/2 turn in one direction. Rotate the brush in the PreservCyt® solution 10 times while pushing against the wall of the ThinPrep® vial. Swirl the brush vigorously to release additional material, then obtain an adequate sample from the ectocervix using a plastic spatula and swirl vigorously in the ThinPrep® vial 10 times. Tighten the cap. For SurePath™, use the cervical broom, rotate five times, place the broom head into CytoRich™ fluid, and tightly cap the vial.
Patient Preparation
Avoid douches 48 to 72 hours prior to examination. Do not collect during or shortly after menstrual period.
Storage Instructions
Maintain at room temperature.
Causes for Rejection
Improper collection; inadequate specimen; improper labeling; specimen more than six months old in ThinPrep® vial; frozen specimen; specimen leaked in transit; quantity not sufficient for analysis; name discrepancies; specimen submitted on male patients; specimen more than four weeks old in SurePath™ vial; excessively bloody specimens.
Other tests from different labs that may be relevant