HIV-1 and HIV-2 Antigen and Antibody Diagnostic Evaluation, Plasma
Use
This test is useful for detecting HIV-1 and HIV-2 infections in symptomatic patients older than 2 years. It serves as follow-up testing for individuals with reactive rapid HIV test results, differentiating between HIV-1 and HIV-2 antibodies. Note that this test should not be used for screening or confirmation in blood donor specimens.
Special Instructions
For diagnostic purposes, results should be considered alongside the patient’s medical history and clinical examination. This test requires appropriate specimen types and handling; it is not licensed for donor screening.
Limitations
A reactive result does not differentiate among HIV-1 p24 antigen, HIV-1 antibody, and HIV-2 antibody. This test's positive predictive value depends on the prevalence of HIV infection in the tested population. Negative results do not rule out acute HIV infection, and repeat testing may be recommended for at-risk individuals. Patients who have undergone prophylactic treatments or recipients of monoclonal antibodies could show false results.
FDA ApprovedNew York Approved
Methodology
Immunoassay (ECLIA)
Biomarkers
LOINC Codes
- 56888-1
- 56888-1
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1.5 mL
Minimum Volume
1 mL
Container
Lavender top (EDTA)
Collection Instructions
Centrifuge blood collection tube per manufacturer's instructions and aliquot plasma into a plastic vial.
Causes for Rejection
Gross hemolysis, gross lipemia, gross icterus, heat-inactivated specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 6 days |
| Frozen | 30 days |