HLA Class I Panel (ABC) by Next Generation Sequencing

Casandra

Casandra Test Code AR33960Version 1 (DRAFT)

Performing Lab

Lab ARUP LaboratoriesLab Test ID 3002307CPT Code 81379

HLA Class I Panel (ABC) by Next Generation Sequencing

Clinical Use

Use

The HLA Class I Panel (ABC) by Next Generation Sequencing is intended for pretransplant allele matching for transplant candidates and their donors. It is designed to identify HLA-A, HLA-B, and HLA-C allelic polymorphisms, providing critical information for matching donors and recipients in transplant medicine. This test is critical for ensuring compatibility and improving graft survival rates in transplant recipients.

Special Instructions

The test is not FDA cleared or approved, but it is performed in a CLIA-certified laboratory at the University of Utah Health. It is important to note that the test should not be used for specific disease screening or diagnosis, such as for celiac disease or rheumatologic diseases. Proper specimen collection and handling are essential, as specimens must be collected in Lavender (K2 EDTA) or Yellow (ACD Solution A) tubes, and should be refrigerated for transport.

Limitations

The test has a high analytical sensitivity and specificity of over 99 percent, but rare diagnostic errors can occur due to primer site mutations. There may also be unknown DNA polymorphisms outside of the regions analyzed, whose significance is not currently known. Furthermore, the test does not have New York state approval, and specimens from New York clients need to be sent to a New York state-approved laboratory.

Test Details