HMB 45
Use
The human melanoma black (HMB) antibody reacts with neuraminidase-sensitive oligosaccharide side chains of glycoconjugates present in immature melanosomes, cutaneous melanocytes, prenatal/infantile retinal pigment epithelium (RPE), and melanoma cells. Studies support the routine use of HMB 45 as a sensitive and specific melanocytic marker.
Special Instructions
Not provided.
Limitations
Specimens must be fixed in 10% NBF or B plus. Fixatives other than specified will be rejected. Stated turnaround times are for clinical use only and may change based on biopharma protocol requirements.
Methodology
Immunoassay (IHC)
Biomarkers
HMB 45
Protein
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED)
Storage Instructions
Specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |