HMB45 Red, technical only
Use
The human melanoma black (HMB) antibody reacts with neuraminidase-sensitive oligosaccharide side chains of glycoconjugates present in immature melanosomes, cutaneous melanocytes, prenatal/infantile retinal pigment epithelium (RPE), and melanoma cells. Studies support the routine use of HMB45 as a sensitive and specific melanocytic marker.
Special Instructions
Not provided.
Limitations
The test does not include a pathologist interpretation; it is the technical component only. Only specimens using 10% NBF or B plus for fixation are accepted to ensure accurate results.
Methodology
Immunoassay (IHC)
Biomarkers
gp100
Protein
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Specimens may be transferred to 70% alcohol for transportation purposes after fixation in 10% NBF or B plus.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |