Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Graphical) With Reflex to HIV-1 GenoSure® MG

Casandra

Casandra Test Code LC44467Version 1 (DRAFT)

Performing Lab

Lab LabcorpLab Test ID 550422CPT Code 87536

Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Graphical) With Reflex to HIV-1 GenoSure® MG

Also known as: HIV-1 RNA by Real-time PCR, Quantitative, Reflex to Genotype, Viral Load

Clinical Use

Order Test

Use

Detect and quantitate HIV-1 in plasma with reflex to genotype (at additional charge) if viral load is >500 copies/mL. GenoSure® MG test detects mutations in the HIV viral genes that encode the HIV protease and polymerase (reverse transcriptase). Mutations in these genes have been associated with resistance to antiretroviral drugs.

Special Instructions

Submit the patient's Social Security number for serial monitoring. To order this test without the serial monitor service, use test 550432. Ensure that the plasma specimen is centrifuged within six hours of collection and frozen immediately.

Limitations

The assay range for this test is between 20 and 10,000,000 copies/mL. Specimens must be handled carefully to avoid rejection due to hemolysis or improper container use. The use of green-top (heparin) tubes is not acceptable and may result in rejection. Specimens must be frozen immediately and shipped in a screw-cap polypropylene frozen transport tube.

Test Details

Methodology

PCR-based (RT-PCR)

Biomarkers

LOINC Codes

Result Codes

20447-9
29541-0
80563-0

Result Turnaround Time

9-16 days

Specimen

Plasma

Volume

6 mL

Minimum Volume

4 mL

Container

Screw-cap polypropylene frozen transport tube

Collection Instructions

Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more screw-cap polypropylene transport tube(s). Snap-cap or pop-top tubes should be avoided and may result in specimen rejection. Immediately freeze specimen at -20° C. Do not place samples on dry ice until completely frozen. Ship specimens frozen.