Human Immunodeficiency Virus 1 (HIV-1), Quantitative, RNA (Abbott® RealTime)
Also known as: HIV-1 Plasma Viremia, HIV-1 RNA by Real-time (RT) PCR, Quantitative, Viral Load, Abbott, Real-time
Use
Detect and quantitate HIV-1 in plasma
Special Instructions
Plasma specimens should be collected in lavender-top (EDTA), PPT™, or yellow-top (ACD-A) tubes. Ensure specimens are centrifuged and plasma is transferred to a transport tube. Specimens must be frozen and shipped on dry ice to maintain stability.
Limitations
The assay can quantitate HIV-1 RNA within a range of 20 to 10,000,000 copies/mL. Results outside this range are not quantitative. The assay does not detect HIV-2 and may not detect all HIV-1 group M subtypes or recombinants.
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 75622-1
- 20447-9
- 29541-0
Result Turnaround Time
3-7 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
2 mL
Minimum Volume
1.1 mL
Container
Screw-cap polypropylene frozen transport tube or centrifuged PPT™ tubes
Collection Instructions
Collect specimen in lavender-top (EDTA), PPT™, or yellow-top (ACD-A) tubes. Centrifuge specimen and transfer plasma to a transport tube. Freeze plasma immediately. Ship frozen on dry ice.
Storage Instructions
Frozen (preferred). Store plasma specimens at -20°C for up to 60 days or at -70°C for up to 6 months for longer storage.
Causes for Rejection
Hemolysis; plasma other than EDTA or ACD-A; quantity not sufficient for analysis
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 72 hours |
| Frozen | -20°C = 60 days, -70°C = 6 months |
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