Human Papillomavirus (HPV) (Aptima®) With Reflex to HPV Genotypes 16 and 18,45
Use
High-risk HPV test is used for types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68, without differentiation of the individual type. If the initial high-risk test is positive, then the residual specimen will be tested for HPV types 16 and 18,45; type 18 cannot be differentiated from type 45.
Special Instructions
Not provided.
Limitations
A negative result from this test does not exclude the possibility of an HPV infection. Factors such as very low levels of the virus or errors in sample collection can lead to false-negative results. Additionally, the test does not differentiate between the individual HPV types in the initial screening. The ability to detect HPV types 16 and 18,45 specifically only occurs if the initial test returns a positive result.
Methodology
PCR-based (Nucleic acid amplification (NAA))
Biomarkers
HPV Genotype 16
MicroorganismHPV Genotype 18,45
MicroorganismHPV high-risk types
Microorganism
LOINC Codes
- 59420-0
- 59420-0
- 77399-4
- 77400-0
- 11502-2
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
ThinPrep® vial
Minimum Volume
Not provided
Container
ThinPrep® vial
Collection Instructions
Broom-like collection technique: Obtain a sample from the cervix using a broom-like device by inserting the brush portion into the cervical os and then rotate the brush five times. Rinse the collection device in the PreservCyt® solution by pushing the brush into the bottom of the vial 10 times, forcing the bristles to bend apart to release the cervical material. As a final step, twirl the brush between the thumb and forefinger vigorously to release additional cellular material. Discard the collection device. Tighten the cap on the ThinPrep® vial so that the torque line on the cap passes the torque line on the vial.
Patient Preparation
Patient should avoid douches 48 to 72 hours prior to examination. Specimen should not be collected during or shortly after menstrual period.
Storage Instructions
Maintain specimen at room temperature. Specimens in ThinPrep® vials must be processed within 21 days of collection for HPV.
Causes for Rejection
Improper collection; inadequate specimen; improper labeling; specimen leaked in transit; quantity not sufficient for analysis; name discrepancies; specimen submitted on male patient; specimen submitted in vial that expired according to manufacturer's label; frozen specimen; SurePath™ vial. For HPV: specimen more than 21 days old in ThinPrep® vial.
Other tests from different labs that may be relevant