IgG4 Immunostain, Technical Component Only
Use
Identification of IgG4-positive plasma cells in the tissue of patients with systemic autoimmune or allergic manifestations. Immunoglobulin G4 is the least abundant of IgG subclasses and elevated serum IgG4 levels may be associated with localized or systemic allergic and autoimmune manifestations. Examples include inflammatory pseudotumor in the liver, breast, and lung, sclerosing pancreatitis, and pemphigus vulgaris, where increased numbers of IgG4-positive plasma cells are present in the tissue.
Special Instructions
Not provided.
Limitations
Age of a cut paraffin section can affect immunoreactivity, and stability thresholds vary widely among published literature, being antigen dependent. The charge of glass slides can be affected by environmental factors and may alter slide staining, leading to inconsistent results due to poor slide surface chemistry. Best practices are to minimize the time slides are stored after being unpackaged, limit exposure to humidity and heat, and minimize exposure to plastics.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
IgG4
Protein
LOINC Codes
- 77202-0 - Laboratory comment Report
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Formalin-fixed, paraffin-embedded tissue block or 2 unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides