Immunoglobulin G (IgG) Heavy and Light Chain (HLC) Pairs, Kappa and Lambda with Ratio
Use
The Immunoglobulin G (IgG) Heavy and Light Chain (HLC) Pairs, Kappa and Lambda with Ratio test is designed to evaluate clonal proliferation of plasma cells and assess the ratios of IgG kappa and lambda. An elevated IgG HLC pair ratio suggests clonal proliferation of an IgG Kappa clone, whereas a low ratio suggests clonal proliferation of an IgG Lambda clone. This can aid in diagnosing and monitoring plasma cell disorders.
Special Instructions
Heavy and light chain (HLC) quantitation should be used as a complementary method to serum protein electrophoresis. Decisions on patient management should include clinical history and other lab findings.
Limitations
This test should not be used alone for making patient evaluation and management decisions. The effect of therapeutic drugs on IgG Kappa and IgG Lambda measurement is not evaluated. Small increases in monoclonal IgG proteins concentrations may not alter HLC pair ratio.
New York Approved
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 74773-3
- 74862-4
- 74863-2
- 74868-1
Result Turnaround Time
6-10 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.75 mL
Minimum Volume
0.5 mL
Container
Plastic vial
Collection Instructions
Centrifuge immediately after coagulation (30 minutes) to prevent hemolysis. Aliquot 0.75 mL of serum into a plastic vial. Send refrigerated.
Causes for Rejection
Hemolysis, lipemia, microbial contamination, particulate matter.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |