Lymphocyte Subset Panel 3 - T-Cell Subsets (CD4 and CD8), Absolute Counts Only
Also known as: ABS
Use
The Lymphocyte Subset Panel 3 measures absolute CD4 and CD8 T-cell counts, which are crucial for categorizing HIV-related clinical conditions according to the CDC's classification system. Monitoring CD4 T-cell levels assists in establishing decision points for the initiation of P. jirovecii prophylaxis and antiviral therapy, as well as in evaluating the efficacy of treatment. CD4 T-cell levels are recommended by the Public Health Service to be monitored every 3 to 6 months in all HIV-infected persons to manage and monitor disease progression and treatment response.
Special Instructions
Specimens must be analyzed within the stability times provided, and any medications that may affect immunophenotyping should be listed on the patient test request form. Specimen collections for longitudinal studies should be performed at the same time of day to maintain consistency.
Limitations
The test is not FDA cleared or approved and is not approved for New York State clients. Care should be taken to ensure specimen integrity, as clotted or hemolyzed samples are unacceptable. The methodology is optimized for whole blood specimens, and additional CBC data is not required for performing the test.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 14135-8
- 24467-3
- 48767-8
- 54218-3
- 8122-4
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
4 mL
Minimum Volume
0.5 mL
Container
Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin) tube
Storage Instructions
Transport at room temperature.
Causes for Rejection
Clotted or hemolyzed specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours for Heparin, 72 hours for EDTA |
| Refrigerated | Unacceptable for both |
| Frozen | Unacceptable for both |
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