MayoComplete Colorectal Cancer Panel, Next-Generation Sequencing, Tumor
Use
The MayoComplete Colorectal Cancer Panel is primarily used for determining patient response to various targeted therapies or immunotherapy and predicting prognosis from microsatellite instability status. This panel evaluates somatic mutations within genes such as APC, BRAF, HRAS, KRAS, MLH1, MSH2, MSH6, NRAS, PIK3CA, PIK3R1, PMS2, POLE, and PTEN to identify candidates for targeted therapy in colorectal cancer patients. Understanding the genetic alterations in these tumors can guide treatment decisions and potentially improve outcomes.
Special Instructions
Not provided.
Limitations
This test does not evaluate for germline alterations within the listed genes. It cannot differentiate between somatic mutations and germline alterations; additional testing may be necessary for hereditary risk assessment. False results may occur if the minimum tumor nuclei requirements are not met or if improper specimen handling occurs. The test is not reliable for detecting multi-exon deletions, larger-scale genomic copy number variants, or epigenetic modifications.
New York Approved
Methodology
NGS (Targeted)
Biomarkers
Microsatellite Instability
Signature
LOINC Codes
- 73977-1 - Cancer multigene analysis Bld/T
- 82939-0 - Genetic variant details Bld/T
- 69047-9 - Geneticist review
- 48767-8 - Annotation comment Imp
- 31208-2 - Specimen source
- 80398-1 - Unique ID Current sample
- 85069-3 - Lab test method
- 62364-5 - Test performance info Spec
- 18771-6 - Provider signing name
Result Turnaround Time
12-20 days
Related Documents
For more information, please review the documents below
Specimen
Tumor Tissue (FFPE)
Volume
Not provided
Minimum Volume
36 mm(2)
Container
Formalin-fixed, paraffin-embedded tissue block or slides
Collection Instructions
Submit a formalin-fixed, paraffin-embedded tissue block or up to 10 unstained slides with 5-micron thick sections of the tumor tissue. Ensure at least 20% tumor nuclei are present.
Causes for Rejection
Improper tissue fixation or decalcification that may cause PCR failure.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 Hours |
| Refrigerated | 5 Days |