Metamycoplasma hominis, Molecular Detection, PCR, Varies
Use
Polymerase chain reaction (PCR) detection of Metamycoplasma hominis is sensitive and specific, providing rapid results. This organism can occasionally be detected in routine plate cultures, which are neither rapid nor sensitive. The PCR assay has replaced conventional cultures due to its speed and equivalent performance. Metamycoplasma hominis is associated with a variety of infections, often as part of the normal genital microbiota. It is implicated in cases such as pelvic inflammatory disease and infections in immunocompromised individuals.
Special Instructions
This test is not intended for medicolegal use. The high sensitivity of the PCR method necessitates processing specimens in a contamination-free environment. Specimen source is required for testing.
Limitations
A negative PCR result does not rule out infection as it may be due to PCR inhibition, primers and probes' sequence variability, or low amounts of Metamycoplasma hominis below the assay's detection limit. Interfering substances or improper specimen handling may affect the assay's accuracy. The test does not detect other Metamycoplasma or Ureaplasma species.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 68546-1
- 31208-2
- 68546-1
Result Turnaround Time
3-4 days
Related Documents
For more information, please review the documents below
Specimen
Buccal Swab
Volume
1 swab
Minimum Volume
1 swab
Container
Culture swab transport system (Dacron or rayon swab)
Collection Instructions
Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.
Causes for Rejection
Cotton or calcium alginate-tipped swab; wooden shaft swab; transport swab containing gel or charcoal.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |