P504S Immunostain, Technical Component Only
Use
Alpha-methylacyl coenzyme A racemase, also known as p504S, is a peroxisomal and mitochondrial enzyme that exhibits a granular expression in the cytoplasm (detected by the chromogen Fast Red). P504S expression is useful in the differentiation between benign prostate glands and prostatic adenocarcinoma as well as in characterizing primary and metastatic renal cell carcinomas.
Special Instructions
Not provided.
Limitations
Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. Best practice is for paraffin sections to be cut within 6 weeks. The charge of glass slides can be affected by environmental factors and subsequently may alter slide staining. Sending unsuitable glass slides can result in inconsistent staining due to poor slide surface chemistry. Best practices for storage of positively charged slides: Minimize time slides are stored after being unpackaged; Limit exposure to high humidity and heat; Minimize exposure to plastics.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Formalin-fixed, paraffin-embedded tissue block OR 2 Unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick
Storage Instructions
Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Causes for Rejection
Wet/frozen tissue, Cytology smears, Nonformalin fixed tissue, Nonparaffin embedded tissue, Noncharged slides, ProbeOn slides, Snowcoat slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |