Parafibromin, Immunostain, Technical Component Only
Use
Parafibromin is a protein encoded by the HRPT2 oncosuppressor gene, and its expression is reported to be decreased or absent in parathyroid carcinomas. Parafibromin is expressed in the nucleus of benign parathyroids, parathyroid adenomas, and other normal tissues. The loss of expression in parathyroid carcinomas makes it a valuable diagnostic tool to identify parathyroid carcinomas and distinguish them from parathyroid adenomas. This test is particularly useful for assisting in the diagnosis of parathyroid carcinomas.
Special Instructions
Not provided.
Limitations
This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for evaluation or a second opinion. Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks. The charge of glass slides can be affected by environmental factors, potentially altering slide staining. Sending unsuitable glass slides can result in inconsistent staining due to poor slide surface chemistry.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Parafibromin
Protein
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Formalin-fixed, paraffin-embedded tissue block preferred or 2 unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides