Parvovirus B19, Molecular Detection, PCR, Plasma
Use
Diagnosing parvovirus B19 infection in plasma specimens. Parvovirus B19 is a DNA virus that preferentially replicates in erythroid progenitor cells. Infection can occur at any age, most common early in life. Transmission is usually by respiratory secretions and occasionally by blood products. Infection can result in asymptomatic courses or diseases ranging from erythema infectiosum in children to severe anemia and systemic manifestations. In pregnant women, it may cause hydrops fetalis, congenital anemia, or stillbirth.
Special Instructions
For ordering: use electronic requests if available, otherwise complete and send the relevant test request forms: Kidney Transplant Test Request or Microbiology Test Request (T244).
Limitations
A negative result does not exclude the presence of parvovirus B19 infection, as viral levels could be below the assay's detection limit or inhibited by substances in the specimen. This test detects only genotype 1 and its effectiveness on genotypes 2 and 3 is unknown. It is not intended to screen asymptomatic individuals and should be interpreted based on consistent clinical signs.
New York Approved
Methodology
PCR-based (qPCR)
Biomarkers
LOINC Codes
- 9571-1
- 9571-1
- 31208-2
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
0.5 mL
Minimum Volume
0.3 mL
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot plasma into a plastic vial.
Storage Instructions
Refrigerated (preferred) for 7 days or frozen for 7 days.
Causes for Rejection
Gross hemolysis
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |