PML::RARA Quantitative, PCR
Use
This test is useful for the diagnosis of acute promyelocytic leukemia (APL), detection of residual or recurrent APL, and monitoring the level of PML::RARA (promyelocytic leukemia/retinoic acid receptor alpha) in APL patients. It involves the detection of the fusion gene PML::RARA which is indicative of neoplastic cells and provides insights into the molecular diagnosis and residual disease monitoring, aiding in treatment approaches for acute myeloid leukemia.
Special Instructions
Refrigerated specimens must arrive within 5 days of collection, while ambient specimens must arrive within 3 days. Collect and package specimens as close to shipping time as possible. The following information is required when submitting: pertinent clinical history, date of collection, and specimen source (blood or bone marrow).
Limitations
The assay may not detect rare, unusual PML::RARA fusions. Accurate comparison of PML::RARA levels requires testing in the same laboratory using the same procedure each time. This assay will only detect PML::RARA RNA and not RNAs from less common RARA fusion genes. It is recommended that changes in positivity status be reconfirmed on a separate specimen.
New York Approved
Methodology
PCR-based (qPCR)
Biomarkers
LOINC Codes
- 31208-2
- 69047-9
Result Turnaround Time
4-8 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
10 mL
Minimum Volume
8 mL
Container
Lavender top (EDTA) or Yellow top (ACD)
Collection Instructions
Invert several times to mix blood. Send whole blood specimen in original tube. Do not aliquot. Label specimen as blood.
Storage Instructions
Refrigerated preferred; ambient acceptable for 72 hours.
Causes for Rejection
Gross hemolysis; moderately to severely clotted.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 3 days |
| Refrigerated | 5 days |