Pneumocystis jirovecii (P. carinii), DFA
Use
Direct immunofluorescence assay (DFA) for detecting Pneumocystis jirovecii (formerly P. carinii), an opportunistic fungal pathogen causing pneumonia especially in immunocompromised patients including those with HIV or post‑transplantation ([jdos.nicholsinstitute.com](https://jdos.nicholsinstitute.com/dos/WyomingMedicalCenter/test/795397?utm_source=openai))
Special Instructions
Client‑prepared slides must be fixed in acetone for 10 minutes prior to drying; client‑prepared tissue slides require director approval. Indicate specimen source in comments, on specimen, and on batch sheet ([jdos.nicholsinstitute.com](https://jdos.nicholsinstitute.com/dos/WyomingMedicalCenter/test/795397?utm_source=openai))
Limitations
Sensitivity of DFA may depend on specimen type and quality, and molecular testing (PCR) is generally preferred in many clinical contexts ([ltd.aruplab.com](https://ltd.aruplab.com/Tests/Pub/0060052?utm_source=openai))
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
3 mL
Minimum Volume
2 mL
Container
Sterile Container
Collection Instructions
Bronchial lavage/wash, sputum, or bronchial alveolar lavage. Bronchial brushings on 2 acetone‑fixed slides acceptable.
Storage Instructions
Keep refrigerated.
Causes for Rejection
Broken slides; slides fixed in formalin; slides fixed in ethanol; unfixed slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 7 Days |
| Frozen | Unacceptable |
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