Powassan Virus, RNA, Molecular Detection, PCR, Spinal Fluid
Use
Rapid testing for Powassan virus RNA (lineage 1 and lineage 2) using cerebrospinal fluid specimens. This test is an adjunctive tool to serology for detecting early Powassan virus infection, particularly useful within the first few days after symptom onset. It should not be used for screening asymptomatic individuals, but for patients exhibiting signs and symptoms of Powassan virus disease.
Special Instructions
The Powassan virus PCR test should not be used as a standalone screening tool for asymptomatic individuals. It is important to use this test in conjunction with clinical findings and other serologic test results to confirm Powassan virus infection.
Limitations
This assay is most useful when used within the first few days of symptom onset. A negative test does not exclude infection with Powassan virus, as the sensitivity decreases after 7 days of illness. This assay detects both viable and nonviable virus, and the performance depends on the viral load present in the specimen, which may not correlate with cell culture results. Improper specimen handling or delayed processing may affect test accuracy.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 34457-2
- 34457-2
- 77202-0
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
1 mL
Minimum Volume
0.3 mL
Container
Sterile vial
Collection Instructions
Send specimen from collection vial 2. Do not centrifuge or heat inactivate.
Causes for Rejection
Heat-inactivated specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |