S-100 Protein by Immunohistochemistry (DAB Detection)

Casandra

Casandra Test Code AR89443Version 1 (DRAFT)

Performing Lab

Lab ARUP LaboratoriesLab Test ID 2004127CPT Code 88342

S-100 Protein by Immunohistochemistry (DAB Detection)

Also known as: S100 IHC

Clinical Use

Order Test

Use

This test provides a technical service-only immunohistochemical stain (DAB detection) for the S‑100 protein, a marker commonly expressed in Schwann cells, melanocytes, chondrocytes, neural tumors, Langerhans cells, and some carcinomas (e.g., breast). It aids in the identification and characterization of such neoplasms through tissue-specific S‑100 expression patterns.

Special Instructions

Submit electronically or use ARUP Immunohistochemistry Stain Form (#32978) if no electronic ordering capability; requires ARUP client number. Transport tissue block or at least 2 positively charged slides (3–5 µm sections) without oven baking; formalin fixation and paraffin embedding required. Ship in cooled container during summer months.

Limitations

This is a stain‑and‑return (technical component only) service; no pathologist interpretation is provided. The test cannot be used to order components directly. Frozen or improperly fixed tissues are unacceptable; depleted or non‑representative specimens are unacceptable. Slides must avoid excessive heat and be freshly cut.

Test Details

New York Approved

Methodology

Immunoassay (IHC)

Biomarkers

S‑100

Protein

IHC • Expression • Ordinal Score (e.g., TPS, CPS, H-score)

LOINC Codes

Result Codes

94736-6

Result Turnaround Time

1-5 days

Specimen

Tissue (FFPE)

Volume

Not provided

Minimum Volume

Not provided

Container

Tissue block or unstained (3‑5 µm) sections on positively charged slides (min: 2 slides)

Collection Instructions

Formalin fix (10% neutral buffered formalin preferred); paraffin embed; cells must be prepared into a cellblock if cells. Protect paraffin from excessive heat; do not oven bake precut slides.