Tryptase Immunostain, Technical Component Only
Use
This test is useful as a marker of mast cells. It supports the diagnosis of mast cell disease due to the characteristic immunohistochemical staining pattern the tryptase antibody produces in mast cells. In patients with suspected mast cell disorders, the staining provides important diagnostic information.
Special Instructions
Not provided.
Limitations
The test is limited by the age of cut paraffin sections, which can affect immunoreactivity. Stability thresholds vary among published literature, and antigen stability is time-dependent, suggesting that paraffin sections should ideally be cut within 6 weeks to avoid diminished staining quality. Environmental factors affecting the charge of glass slides can also alter staining consistency, with improper storage leading to inconsistent results.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Tryptase
Protein
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Attach the green 'Attention Pathology' address label (T498) and the pink Immunostain Technical Only label included in the kit to the transport container.
Storage Instructions
Store positively charged slides by minimizing time unpackaged and limiting exposure to high humidity, heat, and plastics.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin-fixed tissue, nonparaffin-embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |
| Refrigerated | Refrigerated |