Vancomycin-Resistant Enterococcus, Molecular Detection, PCR, Varies
Use
Vancomycin-resistant enterococci (VRE) are major nosocomial pathogens, particularly endangering individuals with hematologic malignancies, liver transplants, or prolonged antibiotic treatment. This test helps identify carriers of VRE by detecting the presence of vanA or vanB genes, which confer resistance. Such detection aids in determining patient isolation requirements and helps prevent nosocomial spread, creating opportunities for cost-effective infection control within healthcare settings.
Special Instructions
The test uses a real-time PCR method to amplify and detect vanA and vanB genes directly from specimens. It requires submission of either perianal, rectal, anal swabs, or fecal specimens for optimal performance. Specimen should be processed to avoid VRE DNA contamination. For those not ordering electronically, a Microbiology Test Request form must accompany the specimen.
Limitations
A positive PCR result indicates colonization with VRE but does not confirm active infection. Similarly, a negative result does not entirely exclude carrier status as low DNA levels or sequence variability can hinder detection. False negatives may occur due to PCR inhibition, necessitating resubmission of a fresh specimen. vanA or vanB occasionally appear in non-enterococcal organisms.
New York Approved
Methodology
PCR-based (Real-Time PCR)
Biomarkers
LOINC Codes
- 62261-3
- 62261-3
- 31208-2
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
Swab
Minimum Volume
Not provided
Container
Culture transport swab (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)
Causes for Rejection
E-swab, calcium alginate or, cotton-tipped swab, Swab sent in gel transport medium, viral or universal transport medium, Dry swab, Formalin or PVA fixative
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |
| Refrigerated | Refrigerated (preferred) |
| Frozen | Frozen |