Von Willebrand Factor VIII
Use
The von Willebrand factor (VWF) is a glycoprotein involved in platelet adhesion and aggregation, and it plays a critical role in hemostasis by stabilizing factor VIII in the blood clotting process. In pathology, vWF is used to assess vascular endothelium and to diagnose bleeding disorders like von Willebrand disease. Additionally, vWF is helpful in identifying endothelial tumors, such as hemangiomas and angiosarcomas.
Special Instructions
Specimens must be tested 3 months after placing in ethanol. Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Stated turn-around-times (TATs) are for clinical use only and subject to change based on biopharma protocol requirements. Final TATs will be specified in the biopharma study contract.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
15°C to 25°C
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |