17-Hydroxyprogesterone 30-Min Quantitative by HPLC-MS/MS, Serum or Plasma
Also known as: OHPRGSTN30
Use
This test measures the levels of 17-Hydroxyprogesterone in serum or plasma using Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS) after 30 minutes of stimulation. It is commonly used in the evaluation and management of congenital adrenal hyperplasia (CAH) or other conditions affecting steroidogenesis. The levels of 17-Hydroxyprogesterone can help in assessing adrenal gland function and identifying enzyme deficiencies.
Special Instructions
This test requires careful patient preparation and specimen collection due to the complexity of hormone assessment post-stimulation. Improperly collected or transported specimens may lead to inaccurate results.
Limitations
This test's reference ranges for 17-Hydroxyprogesterone following stimulation are not well-defined and depend on the stimulation method utilized. Results should be interpreted in the context of the stimulation procedure and clinical findings. The test is not FDA cleared or approved, but developed and its performance characteristics determined by ARUP Laboratories, intended for clinical purposes within a CLIA certified laboratory.
New York Approved
Methodology
Mass Spectrometry
Biomarkers
LOINC Codes
- 48340-4
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.3 mL
Container
ARUP standard transport tube
Collection Instructions
Collect in a serum separator tube. Also acceptable: plain red, pink (K2EDTA), plasma separator tube, green (sodium heparin), or green (lithium heparin).
Storage Instructions
Refrigerated is preferred. Also acceptable: Frozen.
Causes for Rejection
Grossly hemolyzed specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 1 week |
| Frozen | 6 months |