Antimicrobial Susceptibility - Carbapenemase Gene Detection by PCR
Also known as: CARBAR PCR
Use
The Antimicrobial Susceptibility - Carbapenemase Gene Detection by PCR test is designed to detect the presence of predominant carbapenemase genes, such as blaKPC, blaNDM, blaVIM, blaOXA-48, and blaIMP, which are responsible for resistance to carbapenem and other beta-lactam antibiotics. Its primary purpose is to aid infection control in detecting carbapenem-resistant bacteria. However, this test is not intended to guide or monitor treatment of an infection, as a negative result does not exclude other resistance mechanisms or assay-specific nucleic acid in concentrations below the level of detection.
Special Instructions
Not provided.
Limitations
This assay may not detect many variants that diverge from the IMP-1 sequence, such as IMP-2, IMP-7, IMP-8, IMP-13, or IMP-14, which may generate a negative IMP result. The assay may detect IMP-4 at reduced sensitivity and might produce false-negative results for uncommon OXA-48-like variants.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 48767-8 - Annotation comment Imp
- 49617-4 - blaKPC Islt/Spm Ql
- 73982-1 - blaNDM Islt/Spm Ql
- 85498-4 - blaIMP Islt/Spm Ql
- 85501-5 - blaVIM Islt/Spm Ql
- 85503-1 - blaOXA-48 Islt/Spm Ql
- 49617-4 - blaKPC Islt/Spm Ql
- 73982-1 - blaNDM Islt/Spm Ql
- 85503-1 - blaOXA-48 Islt/Spm Ql
- 85501-5 - blaVIM Islt/Spm Ql
- 85498-4 - blaIMP Islt/Spm Ql
- 48767-8 - Annotation comment Imp
Result Turnaround Time
2-3 days
Related Documents
For more information, please review the documents below
Specimen
Cultured Cells
Volume
Not provided
Minimum Volume
Not provided
Container
Sealed container with pure culture on agar slant/bacterial transport media.
Collection Instructions
Collect actively growing Enterobacteriaceae, Pseudomonas aeruginosa, or Acinetobacter baumannii in pure culture. Transport in a sealed container with pure culture.
Storage Instructions
Transport at room temperature.
Causes for Rejection
Mixed cultures or nonviable organisms.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 1 week |
| Refrigerated | 1 week |
| Frozen | Unacceptable |