Antimicrobial Susceptibility - Carbapenemase Gene Detection by PCR
Also known as: CARBAR PCR
Use
The Antimicrobial Susceptibility - Carbapenemase Gene Detection by PCR test is designed to detect the presence of predominant carbapenemase genes, such as blaKPC, blaNDM, blaVIM, blaOXA-48, and blaIMP, which are responsible for resistance to carbapenem and other beta-lactam antibiotics. Its primary purpose is to aid infection control in detecting carbapenem-resistant bacteria. However, this test is not intended to guide or monitor treatment of an infection, as a negative result does not exclude other resistance mechanisms or assay-specific nucleic acid in concentrations below the level of detection.
Special Instructions
An additional processing fee will be billed for all isolates not submitted in pure culture. Isolate identification and specimen source are required. If species identification is not provided, it will be performed at ARUP with additional charges.
Limitations
This assay may not detect many variants that diverge from the IMP-1 sequence, such as IMP-2, IMP-7, IMP-8, IMP-13, or IMP-14, which may generate a negative IMP result. The assay may detect IMP-4 at reduced sensitivity and might produce false-negative results for uncommon OXA-48-like variants.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 48767-8
- 49617-4
- 73982-1
- 85498-4
- 85501-5
- 85503-1
- 49617-4
- 73982-1
- 85503-1
- 85501-5
- 85498-4
- 48767-8
Result Turnaround Time
2-3 days
Related Documents
For more information, please review the documents below
Specimen
Cultured Cells
Volume
Not provided
Minimum Volume
Not provided
Container
Sealed container with pure culture on agar slant/bacterial transport media.
Collection Instructions
Collect actively growing Enterobacteriaceae, Pseudomonas aeruginosa, or Acinetobacter baumannii in pure culture. Transport in a sealed container with pure culture.
Storage Instructions
Transport at room temperature.
Causes for Rejection
Mixed cultures or nonviable organisms.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 1 week |
| Refrigerated | 1 week |
| Frozen | Unacceptable |