Antimicrobial Susceptibility - D-Test (Macrolide, Lincosamide, Streptogramin Resistance)
Also known as: MA DTEST
Use
The Antimicrobial Susceptibility - D-Test is used to determine the inducible resistance of staphylococci, Streptococcus pneumoniae, and beta-hemolytic streptococci to clindamycin. This test helps identify isolates that are presumed to be resistant to clindamycin if inducible clindamycin resistance is detected. It is important in guiding appropriate antibiotic therapy, preventing the use of antibiotics that may be ineffective and ensuring effective patient treatment outcomes.
Special Instructions
An additional processing fee will be billed for all organisms not submitted in pure culture. Isolate identification and specimen source are required for testing. If species identification is not provided, identification will be performed at ARUP with additional charges. Transport specimens at room temperature and in a sealed container with pure culture on an agar slant or in bacterial transport media. Place each specimen in an individually sealed bag.
Limitations
The D-Test provides information on the inducible resistance to clindamycin only and does not cover all possible resistances to other antibiotics. Only pure and viable cultures can be tested; mixed or non-viable cultures are unacceptable and will lead to rejection of the specimen. Incorrect or delayed specimen transport conditions, such as refrigeration or freezing, may affect test results and lead to specimen rejection. False-negative results can occur if the resistance mechanism is not inducible or if the bacterial growth conditions are not optimal.
New York Approved
Methodology
Culture-based
Biomarkers
LOINC Codes
- 42720-3
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Cultured Cells
Volume
Not provided
Minimum Volume
Not provided
Container
sealed container with pure culture on agar slant or in bacterial transport media
Storage Instructions
Transport at room temperature in a sealed container with pure culture. Place each specimen in an individually sealed bag.
Causes for Rejection
Mixed cultures or non-viable organisms.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 1 week |
| Refrigerated | Unacceptable |
| Frozen | Unacceptable |