Babesia microti Antibodies, IgG and IgM by IFA
Also known as: BAB MIC AB
Use
The Babesia microti Antibodies, IgG and IgM by IFA test is primarily used when a Giemsa stain is negative but a high suspicion of babesiosis exists. The test is designed to detect antibodies against Babesia microti specifically, without cross-detecting B. duncani or strain MO-1. Babesia microti infection can lead to babesiosis, a malaria-like disease. Detecting these antibodies at certain levels can indicate either a current or past infection, providing crucial information for diagnosis and management of the disease. Negative results may not necessarily rule out infection during an acute phase, making clinical correlation necessary.
Special Instructions
Specimens should be clearly marked as 'acute' or 'convalescent,' and convalescent specimens should be received within 30 days from the receipt of acute specimens. This distinction is crucial for the accurate interpretation of results related to the infection's timeline. Parallel testing is preferred to improve diagnostic accuracy. Ensure proper labeling and timely separation of serum from cells to avoid specimen rejection.
Limitations
The test will not detect B. duncani or strain MO-1, limiting its scope to Babesia microti. False negatives may occur, especially if the patient is tested too early in the course of infection. Specimens that are bacterially contaminated, hemolyzed, or lipemic are unacceptable and can affect the accuracy of the test results. The test methodology involves semi-quantitative indirect fluorescent antibody (IFA) assessment, and results depend on serum stability and proper specimen handling. Repeated freeze/thaw cycles should be avoided as they may impact the functionality and quantitation of antibodies.
New York Approved
Methodology
Immunoassay (Other)
Biomarkers
LOINC Codes
- 16117-4
- 16118-2
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.4 mL
Container
Serum Separator Tube (SST)
Collection Instructions
Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP standard transport tube. Mark specimens as 'acute' or 'convalescent.'
Causes for Rejection
Bacterially contaminated, hemolyzed, or lipemic specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 2 weeks |
| Frozen | 1 year |