Bordetella pertussis Antibodies, IgA, IgG, and IgM by Immunoblot
Also known as: BORD BLOTS
Use
This test is used to detect and differentiate antibodies (IgA, IgG, and IgM) against Bordetella pertussis, which is the causative agent of whooping cough. Detection of these antibodies is useful for diagnosing recent or previous infections or for assessing vaccination status, particularly in late-stage pertussis cases (>4 weeks). This test is particularly useful when PCR or culture results are negative and there is a high suspicion of pertussis.
Special Instructions
Not provided.
Limitations
This test is not cleared or approved by the FDA and should not be used as the sole basis for diagnosing or ruling out disease. The performance characteristics of this test were determined by ARUP Laboratories, and it is performed in a CLIA-certified laboratory. Interpretive caution is advised as the presence of antibodies can also indicate recent vaccination rather than active infection.
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 93479-4 - Observation Imp
- 74765-9 - B pert.PT IgA Ser Ql Line blot
- 69754-0 - B pert+para.FHA IgG Ser Ql Line blot
- 69755-7 - B pert.PT IgG Ser Ql Line blot
- 74766-7 - B pert.PT 100 IgG Ser Ql Line blot
- 43882-0 - B pert FHA IgM Ser Ql
- 69368-9 - B pert IgM Ser Ql IB
- 69753-2 - B pert+para.FHA IgA Ser Ql Line blot
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.2 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Collect blood in a serum separator tube. Separate serum from cells ASAP or within 2 hours of collection.
Storage Instructions
Refrigerated. Ambient stability: 48 hours; Refrigerated stability: 2 weeks; Frozen stability: 1 year.
Causes for Rejection
Heat-inactivated specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 2 weeks |
| Frozen | 1 year |
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