Bordetella pertussis/parapertussis by PCR
Also known as: BORD PCR
Use
This test is recommended by the CDC for diagnosing pertussis using nasopharyngeal swab and nasal wash specimens. The method employed is qualitative PCR, which is capable of detecting the presence of Bordetella pertussis and Bordetella parapertussis, the bacteria responsible for whooping cough, in respiratory specimens. It provides a reliable means of confirming clinical suspicion of pertussis in patients exhibiting symptoms consistent with this respiratory infection.
Special Instructions
Specimen source must be indicated for processing in the laboratory. Proper collection and handling guidelines should be followed to ensure reliable results. Always place swabs in viral transport media for preservation and transport.
Limitations
A negative test result does not rule out the presence of Bordetella pertussis or Bordetella parapertussis if PCR inhibitors are present in the specimen, or if nucleic acid concentrations are below the detection limits of the assay. Additionally, a false positive for Bordetella parapertussis may occur if the sample contains nucleic acid from Bordetella bronchiseptica.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 23826-1
- 29723-4
- 31208-2
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Nasopharyngeal Swab
Volume
2 mL
Minimum Volume
0.5 mL
Container
Sterile container or viral transport media
Collection Instructions
Transfer aspirated fluid to a sterile container or viral transport media. Swabs should be placed in viral transport media.
Causes for Rejection
Calcium-alginate swabs are unacceptable
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 5 days |
| Frozen | 2 weeks |