Borrelia burgdorferi VlsE1/pepC10 Antibodies, CSF, Total by ELISA With Reflex to IgM and IgG by Immunoblot (Standard Two-Tier Testing, CSF)
Also known as: LYME STTTC
Use
The detection of antibodies to Borrelia burgdorferi in cerebrospinal fluid (CSF) may indicate central nervous system infection. It is a part of the workup for suspected neuroborreliosis. However, the presence of antibodies must be interpreted carefully, considering potential contamination by blood or transfer of serum antibodies across the blood-brain barrier. It is recommended to diagnose Lyme disease in serum before conducting any CSF studies.
Special Instructions
If VlsE1/pepC10 antibodies by ELISA is 0.91 IV or greater, then B. burgdorferi IgG antibody by immunoblot and IgM antibody by immunoblot will be added. Additional charges apply. Specimens from New York clients will be sent out to a New York state-approved laboratory.
Limitations
The test was developed and its performance characteristics were determined by ARUP Laboratories. It has not been cleared or approved by the U.S. Food and Drug Administration. The presence of antibodies in the CSF could be due to contamination or transfer from serum. Lyme disease diagnosis in serum is recommended prior to CSF studies.
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 103961-9
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
6 mL
Minimum Volume
2.5 mL
Container
ARUP standard transport tube
Collection Instructions
Transfer 6 mL CSF to an ARUP standard transport tube. For New York State Clients, transfer 2 mL CSF and 2 mL serum to ARUP standard transport tubes.
Causes for Rejection
Bacterially contaminated, heat-inactivated, hemolyzed, or xanthochromic specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 8 hours |
| Refrigerated | 2 weeks |
| Frozen | 1 month (avoid repeated freeze/thaw cycles) |