Borrelia burgdorferi VlsE1/pepC10 Antibodies, Total by ELISA With Reflex to IgG and IgM by Immunoblot (Standard Two-Tier Testing)
Also known as: LYME STTT
Use
This test is used to diagnose Lyme disease in symptomatic individuals. It is intended for use within four weeks after symptom onset. The reflex testing pattern follows the standard two-tier testing (STTT) approach, where a positive or equivocal ELISA screen is confirmed by immunoblot, enhancing the diagnostic specificity for Lyme disease caused by Borrelia burgdorferi.
Special Instructions
If the VlsE1/pepC10 antibodies by ELISA are 0.91 IV or greater, the B. burgdorferi IgG antibody by immunoblot and IgM antibody by immunoblot will be added, resulting in additional charges.
Limitations
Negative results may occur in recently infected patients (less than 14 days). Equivocal results on the ELISA, with values between 0.91 - 1.09 IV, should be followed by repeat testing in 10-14 days if disease is still suspected. Plasma, CSF, and specimens that are contaminated, heat-inactivated, hemolyzed, icteric, or lipemic are unacceptable for this test and can lead to inaccurate results.
New York Approved
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 100711-1
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
2 mL
Minimum Volume
0.15 mL
Container
ARUP standard transport tube
Collection Instructions
Collect in serum separator tube and transfer specified volume to transport tube.
Storage Instructions
Refrigerate the specimen for transport.
Causes for Rejection
Plasma, CSF, contaminated, heat-inactivated, hemolyzed, icteric, or lipemic specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 10 days |
| Frozen | 1 month |