Borrelia Species by PCR (Lyme Disease), Tick
Also known as: LYME TICK
Use
This test is used for the detection of Borrelia species, which are causative agents of Lyme disease. The test employs qualitative real-time polymerase chain reaction (PCR) methodology to detect Borrelia spp. DNA from tick specimens. It is utilized as an adjunct to clinical findings for the investigation of potential Lyme disease exposure in individuals who may have been bitten by deer ticks.
Special Instructions
The test is conducted at Quest Diagnostics and is not FDA cleared or approved. It has been validated according to CLIA regulations for clinical purposes. Specimens must be transferred in ethanol and stored under specified conditions. It is advised not to perform tick testing as a standalone diagnostic tool for Lyme disease.
Limitations
The test is limited to identifying DNA from Borrelia species in tick specimens and should be interpreted in conjunction with clinical signs and symptoms. Submission in formalin is unacceptable as it may hamper PCR detection. The test’s utility is restricted to environmental surveillance and not direct patient testing for clinical diagnosis.
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 90042-3
- 90042-3
Result Turnaround Time
3-6 days
Related Documents
For more information, please review the documents below
Specimen
Body Fluid
Volume
Not provided
Minimum Volume
Not provided
Container
ARUP Standard Transport Tube
Collection Instructions
Transfer one deer tick in 70 percent ethanol.
Storage Instructions
Refrigerated.
Causes for Rejection
Specimens submitted in formalin.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 1 week |
| Frozen | 1 month |
