CD8 by Immunohistochemistry
Also known as: CD8 IHC
Use
CD8 by Immunohistochemistry (IHC) is a technical stain-and-return service performed Monday through Friday, intended to detect and visualize CD8 protein expression in tissue or cell-block specimens prepared from formalin-fixed, paraffin-embedded blocks or unstained slides. It serves as an immunohistochemical method to assist in the histopathologic evaluation of specimens through detection of CD8-positive cells.
Special Instructions
Submit electronic order requests. If unavailable, use ARUP Immunohistochemistry Stain Form (#32978). Refer to ARUP Immunohistochemistry Stain Offerings brochure. Transport specimens using ARUP supply kit #47808. Protect blocks/slides from excessive heat. If sending precut slides, do not oven bake. Ship in cooled containers during summer. The stain-and-return service is technical only.
Limitations
Specimens must be formalin-fixed (10 percent neutral buffered formalin) and paraffin-embedded, or cells prepared into a cell block. Unacceptable specimens include depleted specimens or those with non-representative tissue. Frozen specimens are unacceptable. Slides must be positively charged, 3–5 µm thick; minimum of 2 slides required. Stability: ambient and refrigerated are indefinite; frozen specimens are unacceptable.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 94736-6
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
tissue block transport kit or 5 unstained positively charged slides
Collection Instructions
Transport tissue block or 5 unstained (3‑ to 5‑micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808). If sending precut slides, do not oven bake.
Storage Instructions
Room temperature or refrigerated; ship in cooled container during summer months.
Causes for Rejection
Depleted specimens; specimens with non‑representative tissue type.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |