Coccidioides by PCR
Also known as: COCCI PCR
Use
The Coccidioides by PCR test is a qualitative assay designed to aid in the diagnosis of coccidioidomycosis, also known as Valley Fever. This infection is caused by the fungi Coccidioides immitis and Coccidioides posadasii. The test detects the presence of these fungi in respiratory samples, cerebrospinal fluid (CSF), and tissue specimens. This test serves as an adjunct to culture and serology but is not a replacement for these methods. Early detection and diagnosis are crucial for effective management and treatment of the disease, especially in regions where these fungi are endemic.
Special Instructions
Specimen source information is required at the time of submission for accurate processing and interpretation. It is important that tissue specimens are frozen immediately after collection to maintain integrity. Frozen transport conditions must be adhered to for all specimen types.
Limitations
While this PCR test is effective for detection, it does not distinguish between C. immitis and C. posadasii. Negative results do not rule out the presence of PCR inhibitors or nucleic acid concentrations below the detection level of the assay. Paraffin-embedded tissues and certain specimen types such as serum, plasma, and whole blood are not acceptable for this assay. The presence of inhibitors or improper storage can also affect the accuracy of results.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 66746-9
- 97916-1
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Bronchoalveolar Lavage
Volume
1 mL
Minimum Volume
0.5 mL
Container
Sterile container
Collection Instructions
Transfer bronchoalveolar lavage (BAL) to a sterile container.
Storage Instructions
Freeze immediately for tissue specimens.
Causes for Rejection
Paraffin-embedded tissue, serum, plasma, whole blood
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days for respiratory and CSF, unacceptable for tissue |
| Refrigerated | 14 days |
| Frozen | 14 days |