Cyclosporine A, 2-Hour Post Dose (C2) by Tandem Mass Spectrometry
Also known as: C2CYA
Use
Cyclosporine A levels measured two hours post-dose may provide a better estimate of the area under the concentration-time curve (AUC) compared to trough levels. This test is instrumental in optimizing drug dosage and monitoring patient adherence to therapy. It is crucial for determining therapeutic ranges depending on various factors, such as indication for therapy, treatment phase (initiation or maintenance), and the therapeutic approach of the transplant center. Suggested target ranges are specified for renal (800-1700 ng/mL) and liver (600-1000 ng/mL) transplants, varying post-transplant and during maintenance phases.
Special Instructions
Cyclosporine whole blood concentrations can be determined using chromatographic or immunoassay methodologies. Different methodologies may yield varying results due to differences in detection, necessitating consideration of the method used when interpreting results.
Limitations
The test results can vary significantly based on the chosen assay methodology, which must be considered when interpreting cyclosporine levels. Immunoassays are known to have a positive bias compared to HPLC-MS/MS assays due to potential antibody cross-reactivity with cyclosporine metabolites, impacting reference range applicability.
New York Approved
Methodology
Mass Spectrometry
Biomarkers
LOINC Codes
- 32997-9
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
1 mL
Minimum Volume
0.25 mL
Container
Lavender (EDTA) or pink (K2EDTA).
Patient Preparation
Two hour post-dose level should be drawn.
Storage Instructions
Refrigerated.
Causes for Rejection
Serum or plasma. Specimens left at room temperature for longer than 24 hours. Clotted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 2 weeks |
| Frozen | 2 months |