Cyclosporine A by Tandem Mass Spectrometry
Also known as: CYA
Use
Cyclosporine A by Tandem Mass Spectrometry is used to optimize dosing and monitor patient adherence, especially important in individuals who have undergone organ transplants. This test measures the concentration of Cyclosporine A in whole blood, which helps in maintaining the therapeutic range for immunosuppressive therapy. Keeping within the therapeutic range is crucial for preventing organ rejection and minimizing side effects. The therapeutic range varies depending on the type of transplanted organ and the time elapsed post-transplantation.
Special Instructions
Cyclosporine concentrations can be measured using different methodologies, such as chromatographic or immunoassay. These methods are not interchangeable, and results should not be compared directly across different testing platforms. Reference ranges may vary due to methodology differences, particularly due to cross-reactivity in immunoassays.
Limitations
This test does not differentiate between Cyclosporine A metabolites and other similar compounds, which can lead to variability in results when different methodologies are used. Immunoassays, for example, may have a positive bias compared to HPLC-MS/MS due to cross-reactivity, potentially affecting the interpretation of therapeutic ranges and adherence monitoring.
New York Approved
Methodology
Mass Spectrometry
Biomarkers
LOINC Codes
- 3520-4
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
1 mL
Minimum Volume
0.25 mL
Container
Lavender (EDTA) or pink (K2EDTA)
Collection Instructions
Predose (trough) levels should be drawn.
Storage Instructions
Refrigerated transport.
Causes for Rejection
Serum or plasma. Specimens left at room temperature for longer than 24 hours. Clotted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 2 weeks |
| Frozen | 2 months |