Direct Immunofluorescence, Tissue Biopsy (Cutaneous, Mucosal, Epithelial)
Also known as: CUTDIF
Use
This test is used for the assessment of pruritic, urticarial, blistering, and/or erosive inflammatory conditions, including immune-mediated cutaneous diseases. Direct Immunofluorescence is particularly useful in diagnosing immunobullous diseases, epithelial antibody-associated disorders, and other inflammatory skin conditions by detecting specific immunoglobulin deposits in biopsy specimens.
Special Instructions
Not provided.
Limitations
Direct Immunofluorescence may not distinguish between certain pemphigoid variants or differentiate specific immunological markers in isolation; additional serum testing or alternative fixation methods may be required for comprehensive analysis. The fluorescence fades over time, limiting long-term specimen visualization. Negative control sections and technical adequacy checks are essential for ensuring valid results.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 6471-7 - Other Stn Tiss
Result Turnaround Time
3-9 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Unknown)
Volume
Not provided
Minimum Volume
Not provided
Container
Michel's medium or Zeus tissue fixative container
Collection Instructions
Transport tissue (optimal 4-6 mm) in Michel's medium, available through ARUP supply or call ARUP Client Services.
Causes for Rejection
Formalin-fixed tissue, frozen in Michel's medium, tissue in unknown or unacceptable medium, tissue sections on slides.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 10 days |
| Refrigerated | 10 days |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant