Hepatitis Delta Virus by Quantitative PCR
Also known as: HDV QNT
Use
This test is designed to confirm and quantify the presence of hepatitis D virus (HDV) in serum samples. It is useful for assessing the viral load in patients known to be infected with HDV, which is critical for disease management and monitoring the effectiveness of antiviral therapy. The test uses quantitative polymerase chain reaction (PCR) to measure HDV RNA levels, providing a precise quantification of viral burden. Accurate measurement of viral load aids physicians in tailoring treatment regimens and monitoring disease progression.
Special Instructions
Not provided.
Limitations
The test has a quantitative range from 2.0 to 6.7 log IU/mL (92 to 4,600,000 IU/mL). Results less than 2.0 log IU/mL or 92 IU/mL are considered negative, but may not rule out the presence of PCR inhibitors or HDV RNA concentrations below the detection threshold. Inhibition in samples could lead to underestimation of viral quantitation, potentially affecting the accuracy of the results. Additionally, only HDV genotype 1 was validated through wet bench studies, while other genotypes were validated in silico, limiting the study accuracy for those variants.
New York Approved
Methodology
PCR-based (qPCR)
Biomarkers
Hepatitis Delta Virus
Microorganism
LOINC Codes
- 31208-2 - Specimen source
- 85512-2 - HDV RNA SerPl NAA+probe-aCnc
- 85513-0 - HDV RNA Ser NAA+probe-Log IU
- 7906-1 - HDV RNA SerPl Ql NAA+probe
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
2 mL
Minimum Volume
0.5 mL
Container
sterile container
Collection Instructions
Separate serum from cells before transport.
Storage Instructions
Transport frozen.
Causes for Rejection
Improperly preserved specimens or incorrect transport conditions.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 1 week |
| Frozen | 30 days |
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