Herpes Simplex Virus DFA with Reflex to Herpes Simplex Virus Culture
Also known as: HSVFAC
Use
The Herpes Simplex Virus DFA with Reflex to Herpes Simplex Virus Culture test is designed for the rapid identification of herpes simplex virus (HSV) infections primarily in active lesions, such as vesicles, ulcers, and mucous membrane inflammation. It is particularly beneficial in clinical settings where differentiating between HSV infections and other conditions is critical for patient care and disease management. Despite the availability of molecular testing methods, this culture-based approach remains a reliable option when immediate results are necessary and where molecular testing may not be feasible.
Special Instructions
Not provided.
Limitations
The sensitivity of the DFA methodology depends significantly on the quality and adequacy of the specimen. If the specimen contains fewer than 20 cells, the DFA result will be reported as 'inadequate,' necessitating a reflex to culture for accurate results. Additionally, the test does not differentiate between HSV-1 and HSV-2 beyond the primary detection phase, as it primarily confirms the presence of the virus using culture techniques.
New York Approved
Methodology
Cell-based / Cytometry
Biomarkers
LOINC Codes
- 5846-1 - HSV1 Ag Spec Ql IF
- 5849-5 - HSV2 Ag Spec Ql IF
- 31208-2 - Specimen source
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Saliva
Volume
3 mL
Minimum Volume
0.5 mL
Container
Sterile container or viral transport media (ARUP Supply #12884)
Collection Instructions
Collect from buccal mucosa lesion, cervical or genital lesion, eye, throat, or vesicle swab; bronchoalveolar lavage (BAL); tissue or vesicle fluid.
Storage Instructions
Refrigerated.
Causes for Rejection
Bone marrow, CSF, or whole blood. Calcium alginate, eSwab, dry or wood swabs.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 4 hours |
| Refrigerated | 72 hours |
| Frozen | Unacceptable |