IGH-BCL2 Fusion, t(14;18) by FISH
Also known as: BCL2_FISH
Use
The IGH-BCL2 Fusion, t(14;18) by FISH test is designed to detect the IGH-BCL2 fusion associated with translocation t(14;18)(q32;q21). This fusion is found in a variety of B-cell lymphomas, including follicular lymphomas, diffuse large B-cell lymphomas (DLBCL), and 'double hit' or 'triple hit' lymphomas. The detection of this fusion by FISH allows for the assessment of abnormal signal patterns that signify the presence of the fusion, providing critical information that can be correlated with clinical, morphologic, and immunophenotypic data to assist in diagnosis and treatment planning for mature B-cell lymphomas.
Special Instructions
Not provided.
Limitations
The test is designed to detect the IGH-BCL2 fusion with 100% sensitivity in patients with IGH-BCL2-rearranged lymphomas, excluding rare cryptic rearrangements. Assay performance and limits of detection were validated using normal and known positive cases, but the test's performance characteristics for unexpected or novel BCL2 fusions are not determined.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
DYNLT3-BIK fusion
Fusion
LOINC Codes
- 89873-4 - Unique ID Initial sample
- 31208-2 - Specimen source
- 77038-8 - t(14;18)(IGH,BCL2) Bld/T FISH
- 81304-8 - Struct var analysis method
- 78236-7 - Cells.IGH rear/Cells cnt NFr Bld/T FISH
Result Turnaround Time
3-7 days
Related Documents
For more information, please review the documents below
Specimen
Tumor Tissue (FFPE)
Volume
Not provided
Minimum Volume
2 slides or tissue block
Container
Paraffin block or 5 unstained positively charged slides
Collection Instructions
Formalin fix with 10 percent neutral buffered formalin and paraffin embed. Transport tissue block or slides in a tissue transport kit (ARUP supply #47808).
Storage Instructions
Room temperature or refrigerated. Ship in cooled container during summer months.
Causes for Rejection
Paraffin block with no tumor tissue remaining, non-formalin fixed specimens, decalcified specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |