IGH-MYC Fusion t(8;14) by FISH
Also known as: IGHMC FISH
Use
This test is designed to detect the IGH-MYC fusion associated with the chromosomal translocation t(8;14)(q24;q32), which is often present in B-cell lymphomas, including Burkitt lymphoma and diffuse large B-cell lymphoma (DLBCL). It helps in diagnosing Burkitt lymphoma and B-cell lymphoma with features intermediate between BL and DLBCL. The test uses differentially labeled fluorescent probes to identify fusion signals, which, if present in 21% or more of cells, indicate a positive result.
Special Instructions
Not provided.
Limitations
The test is expected to detect IGH-MYC rearrangements in patients with IGH-MYC-rearranged lymphomas, except for rare instances of cryptic rearrangements. The assay range and limit of detection were generated using normal and known positive cases. The test was developed by ARUP Laboratories and is not cleared or approved by the FDA, although it is performed in a CLIA certified laboratory and intended for clinical purposes.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
IGH, MYC
GeneChromosome Chromosome 8
Chromosome
LOINC Codes
- 81304-8 - Struct var analysis method
- 78236-7 - Cells.IGH rear/Cells cnt NFr Bld/T FISH
Result Turnaround Time
3-7 days
Related Documents
For more information, please review the documents below
Specimen
Tumor Tissue (FFPE)
Volume
Not provided
Minimum Volume
2 slides
Container
Tissue transport kit
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin-embed specimen. Transport tissue block or 5 unstained (3-micron thick sections) positively charged slides in a tissue transport kit.
Storage Instructions
Room temperature or refrigerated. Ship in cooled container during summer months.
Causes for Rejection
Paraffin block with no tumor tissue remaining. Specimens fixed in any fixative other than 10 percent neutral buffered formalin. Decalcified specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |
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