Insulin, Other
Also known as: INSULINOTH
Use
This test is used to measure insulin levels in the blood. It provides valuable information for assessing insulin levels in patients, which can aid in the diagnosis and management of conditions related to insulin production and function, such as diabetes mellitus. The test measures the amount of insulin in the serum and reacts on a nearly equimolar basis with several insulin analogs: insulin aspart, insulin glargine, and insulin lispro, with insulin detemir showing approximately 50% cross-reactivity. Test reactivity with insulin glulisine is negligible (< 3 percent).
Special Instructions
Patients should be aware that the test requires collection in a Serum Separator Tube (SST). After the sample clots at room temperature, the serum must be separated from the cells as soon as possible and transferred to an ARUP Standard Transport Tube. The test should be ordered considering its limitations in cross-reactivity with different insulin analogs.
Limitations
The test has not established a reference interval and exhibits various levels of cross-reactivity with insulin analogs. Users should note the specific reactivity: insulin aspart, insulin glargine, and insulin lispro react on a nearly equimolar basis, while insulin detemir shows about 50% cross-reactivity. Insulin glulisine shows negligible reactivity, which may impact the interpretation when these analogs are present. Furthermore, interpretations should consider the conversion factor of µIU/mL to pmol/L by multiplying by 6.0.
FDA ApprovedNew York Approved
Methodology
Immunoassay (CLIA)
Biomarkers
LOINC Codes
- 20448-7
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.4 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Allow sample to clot completely at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube.
Storage Instructions
Transfer separated serum to ARUP Standard Transport Tube and freeze.
Causes for Rejection
Vitreous fluid, gray (sodium fluoride/potassium oxalate) or heparinized plasma, hemolyzed specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 8 hours |
| Refrigerated | 1 week |
| Frozen | 1 month |