Legionella Species by Qualitative PCR
Also known as: LEGIONPCR
Use
This test is designed to detect the presence of Legionella species in respiratory specimens. It is particularly useful for diagnosing infections caused by these bacteria, such as Legionnaires' disease and non-differentiable species such as L. pneumophila. Detection of Legionella DNA aids in the early diagnosis and management of community-acquired pneumonia associated with these pathogens.
Special Instructions
Specimen source must be indicated when submitting samples. Tissues containing optimal cutting temperature compound are unacceptable. Contact ARUP Client Services for information on transport media availability.
Limitations
A negative test result does not definitively exclude the presence of Legionella species as inhibitors in the specimen or DNA concentrations below the detection threshold can result in false negatives. The test cannot differentiate between Legionella species, and its clinical significance is currently undetermined for non-pneumophila species. The test has not been FDA cleared or approved and is primarily intended for clinical use in a CLIA certified laboratory.
New York Approved
Methodology
PCR-based (Qualitative Polymerase Chain Reaction)
Biomarkers
LOINC Codes
- 31208-2
- 21363-7
- 101219-4
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Bronchoalveolar Lavage
Volume
2 mL
Minimum Volume
0.5 mL
Container
Sterile container
Collection Instructions
Transfer respiratory fluid to a sterile container or viral transport media
Storage Instructions
Freeze samples for transport.
Causes for Rejection
Specimens containing tissues in optimal cutting temperature compound.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 5 days |
| Frozen | 6 months |