MDM2 Gene Amplification by FISH
Also known as: MDM2_FISH
Use
MDM2 Gene Amplification by FISH is utilized to aid in the differential diagnosis between well-differentiated liposarcoma and benign lipoma. This differentiation is particularly important for individuals who are diagnosed or suspected of having well-differentiated liposarcoma based on tissue morphology. The test assesses the MDM2 amplification status using FISH analysis on paraffin-embedded tumor tissue, targeting the MDM2 gene and the chromosome 12 centromere (CEP12).
Special Instructions
Formalin fix tissue with 10 percent neutral buffered formalin, and paraffin-embed before shipping. Transport in a tissue transport kit and ensure slides are positively charged. Multiple specimens require specific ordering notes or will incur additional fees if not clarified.
Limitations
Limited tissue samples, tumor heterogeneity, low neoplastic cell burden, or heavily inflamed tumors may affect assay sensitivity, potentially leading to false-negative results. Interpretation should consider clinical and histopathologic findings. MDM2 amplification may also appear in non-adipocytic tumors and other tumors with lipomatous differentiation.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
LOINC Codes
- 81304-8
- 78236-7
Result Turnaround Time
3-7 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
2 slides
Container
Paraffin block or 5 unstained positively charged slides
Collection Instructions
Formalin fix with 10 percent neutral buffered formalin and paraffin-embed tissue. Ship in a tissue transport kit.
Storage Instructions
Room temperature or refrigerated. Ship in cooled container during summer months.
Causes for Rejection
Paraffin block with no tumor tissue, non-formalin fixatives, decalcified specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |