Mycoplasmoides genitalium Detection and Macrolide Resistance by PCR
Also known as: MACRO PCR
Use
This test is used to detect Mycoplasmoides (Mycoplasma) genitalium infection and predict resistance to macrolide antibiotics. Macrolide drug resistance is predicted based on melt probe analysis, focusing on common resistance-associated mutations at positions A2058, A2059, and A2062 of the 23S rRNA gene. The test is designed for clinical use to aid in the management of infections and antibiotic treatment decisions.
Special Instructions
Not provided.
Limitations
While the test predicts macrolide resistance by detecting known mutations, it does not account for all possible resistance mutations. Mutations of unknown significance may be detected, indicating the potential presence of macrolide resistance that was not accounted during development. A negative result cannot rule out the presence of PCR inhibitors or detect nucleic acid at concentrations below the assay's level of detection.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 31208-2 - Specimen source
- 91849-0 - M genitalium DNA Genital Ql NAA+probe
Result Turnaround Time
2-7 days
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
1 mL
Minimum Volume
0.5 mL
Container
Viral transport media
Collection Instructions
Transfer swab or 1 mL urine to viral transport media available from ARUP supply.
Storage Instructions
Frozen storage is required for transport.
Causes for Rejection
Specimen without specimen source identification.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 10 days |
| Frozen | 14 days |
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