p53 with Interpretation by Immunohistochemistry
Also known as: P53 IP
Use
Abnormalities of the p53 gene are one of the most common genetic changes associated with cancer and can be found in a wide variety of tumor types. p53 protein expression is generally associated with a worse prognosis. According to some studies, nuclei staining in less than 10 percent of specimen cells is defined as negative for p53, and nuclei staining in greater than or equal to 10 percent of specimen cells is considered positive for p53. Results of this test should be interpreted with caution and within the context of other clinical data.
Special Instructions
Not provided.
Limitations
The test results should be interpreted with caution and within the context of other clinical data. Any specimens fixed in a fixative other than 10 percent neutral buffered formalin, as well as decalcified specimens, are unacceptable. Unacceptable conditions also include paraffin blocks with no tumor tissue remaining.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
p53
Protein
LOINC Codes
- 31149-8 - P53 protein Ag NFr Tiss ImStn
- 94736-6 - Tissue block ID Spec
- 31208-2 - Specimen source
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Tumor Tissue (FFPE)
Volume
Not provided
Minimum Volume
3 slides
Container
Tissue block or 5 unstained slides in a tissue transport kit
Collection Instructions
Formalin fix (10 percent neutral buffered formalin is preferred) and paraffin embed specimen. Protect paraffin block and/or slides from excessive heat. Transport tissue block or positively charged slides in a tissue transport kit.
Storage Instructions
Room temperature or refrigerated. Ship in cooled container during summer months.
Causes for Rejection
Paraffin block with no tumor tissue remaining. Specimens fixed in any fixative other than 10 percent neutral buffered formalin. Decalcified specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |